1. Uniform detection of 15'000+ genes at 1 million reads per sample across 96 samples
Distribution of the number of detected genes across 96 blood RNA samples prepared with the MERCURIUS™ Blood BRB-seq library preparation kit. The library was sequenced at an average of 1 million reads per sample on an Illumina NextSeq 550.
2. The Blood BRB-seq provides state-of-the-art performance in terms of globin depletion efficiency
(left) Proportion of mapped reads assigned to globin genes (blue) and other genes (grey) for blood RNA samples without globin depletion and with globin depletion performed using ThermoFisher’s GLOBINclear™-Human Kit and the Blood BRB-seq method. (right) Number of detected genes across the three different conditions. Non-depleted libraries show a sharp decrease in performance, while BRB-seq and Globin Clear libraries both provide rich globin-free data.
3. Globin depletion is effective and specific to the target globin genes
Correlation plots between mapped reads in between non-depleted libraries and Blood BRB-seq (left) and Globin Clear-depleted libraries (right). For both methods, globin depletion is effective and specific to the target globin genes, with negligible off-target effects on other genes.
4. Blood BRB-seq provides effective and high-throughput inline depletion of globin genes
(left) Read distribution to the main globin genes on BRB-seq libraries WITHOUT globin depletion. The majority of mapped genes are associated with globin genes. (right) Read distribution to the main globin genes on BRB-seq libraries WITH globin depletion. The reads associated to globin genes are drastically reduced.
For (application): 3' mRNA sequencing
For use with (equipment): Illumina and AVITI NGS instruments
Species compatibility: Human and mouse
Available formats: 96, 384 and 1'536 preps
Shipping conditions: Dry ice
Storage conditions: -20C
