1. Detection of 12'000+ genes at 1.5 million reads per sample across 384 samples
Distribution of the number of detected genes across 384 samples prepared with the MERCURIUS™ DRUG-seq library preparation kit from frozen cells. The library was sequenced at an average of 1.5 million reads per sample on an Illumina NovaSeq 6000.
2. DRUG-seq shows high demultiplexing rate and high mapping rate to exons
DRUG-seq performance shows 97% demultiplexing rate from raw data and 85% mapping rate of the 384 pooled samples, sequenced at 1.5 million reads per sample.
3. DRUG-seq shows a comparable number of detected genes at different cell seeding densities
The number of detected genes was assessed for three different numbers of cells per well: 1k, 5k, and 100k cells per well. The libraries were then sequenced at 1.5 million reads per sample.
4. Comparable number of detected genes between the DRUG-seq and BRB-seq protocol
The number of detected genes was assessed between the DRUG-seq protocol, RNA extraction-free: directly from frozen cell lysates, and the BRB-seq protocol, from purified RNA. Each library contains 24 samples, sequenced at aprox 2M reads/sample.
5. Library performance is comparable between the DRUG-seq and BRB-seq protocol
Both libraries, DRUG-seq from frozen cell lysates, and BRB-seq from purified RNA, show a very high demultiplexing rate, above 99% and a mapping rate above 76%.
For (application): 3’ mRNA sequencing
For use with (equipment): Illumina, MGI and AVITI NGS instruments
Species compatibility: All eukaryotic species
Available formats: 96, 384 preps 1'536 preps
Shipping conditions: Dry ice
Storage conditions: -20C
