MercuriusTM
RNA-seq library preps directly from organoid lysates without prior RNA isolation.
Compatible with Illumina® and AVITI™.
Catalog number |
#10861 |
#11061 |
#10871 |
#11071 |
Kit size |
96 preps |
4x96 preps |
384 preps |
4x384 preps |
Total preps (how many library preps can be prepared in total with one kit) |
96 |
384 |
384 |
1,536 |
Sample multiplexing and plate format (how many samples can be multiplexed in one single tube) |
96 |
96 |
384 |
384 |
Barcoded oligo-dT plates included |
1 |
4 |
1 |
4 |
UDI pairs included (how many different libraries can be sequenced together) |
4 |
4 |
4 |
4 |
The MERCURIUS™ Extraction-free Organoid DRUG-seq kits contain all the oligos and enzymes needed to go from 3D organoids and spheroids to sequencing-ready libraries
More samples, more replicates. Robust results, significant discoveries.
An optimized lysis buffer for complete lysis and efficient reverse transcription.
Without pre-amplification, leading to higher mapping and gene detection rates.
Convenient and short protocol from samples to sequencing-ready libraries in one day.
For (application): 3’ mRNA sequencing
For use with (equipment): Illumina and AVITI NGS instruments
Species compatibility: All eukaryotic species
Available formats: 96, 384 and 1'536 preps
Shipping conditions: Dry ice
Storage conditions: -20C
Number of samples:
12
Reads per sample in demo dataset:
10'000 reads
To have access to the deep-sequenced dataset (24.5M reads per sample) contact us.
Demo dataset file size:
7.16 MB
Each DRUG-seq or BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools. To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
The recommended range of input material is in the range of 5’000-50’000 cells.
The only difference between DRUG-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the DRUG-seq barcode sequence. For a thorough description of DRUG-seq data processing, please refer to the DRUG-seq kit user guide.
The barcode set for your kit is conveniently located on the kit label. Please refer to the label for accurate identification. For optimal compatibility, ensure that you use the appropriate plate format (e.g., for kits designed for 96 reactions, the 96 well-plate format should be used). This ensures accurate and efficient processing of your samples. If you have any further questions or concerns, please contact our support team for assistance by email or using our live chat tool.
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Our Organoid DRUG-seq service delivers raw sequencing data (fastq files), gene count matrices and analysis report files. A cost-efficient option suitable for projects of all sizes.
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