1. Uniform detection of 22'000+ genes at 12 million reads per sample across 48 samples
Distribution of the number of detected genes across 48 samples prepared with the MERCURIUS™ Multiplexed RNA-seq (mRNA) service. The library was sequenced at an average of 12 million reads.
2. Multiplexed RNA-seq (mRNA) shows high demultiplexing rate and high mapping rate to exons
Multiplexed RNA-seq (mRNA) performance shows 99% demultiplexing rate from raw data, 78% mapping rate, and 18% duplication rate of the 48 pooled samples and sequenced at 12 million reads per sample.
3. Mapping rates as high as 80% for different RNA inputs
Mapping rates are uniform across three RNA inputs tested: 1ug, 10ng, and 100ng. Human Universal Reference RNA was used to prepare mRNA Multiplexed RNA-seq libraries.
4. Multiplexed RNA-seq exhibits reads distribution across the transcript’s entire length
The gene body coverage shows a consistent and uniform reads distribution across the entire gene body for the Multiplexed RNA-seq (mRNA) protocol, comparable to competitor N protocol, while the BRB-seq protocol shows a significant 3' bias due to its poly-A selection methodology.
5. >50'000 isoforms detected at 12M sequencing depth
Benchmarking the Multiplexed RNA-seq (mRNA) protocol against competitor N shows similar performance for isoform detection. More than 50,000 isoforms can be detected at a sequencing depth of 12M reads per sample.
For (application): Full-length mRNA sequencing
For use with (equipment): Illumina and AVITI NGS instruments
Species compatibility: All eukaryotic species
Available formats: 96 and 384 preps
Shipping conditions: Dry ice
Storage conditions: -20C
